ESDAR Conference 2018

ESDAR Scientific Programme

Thursday 27th September 2018

Pre-Conference Sessions

Practical Parallel Sessions

Veterinary Teaching Hospital, Faculty of Veterinary Medicine



1 Equine oocyte collection

Katrin Hinrichs (Texas, A&M University, USA)


2 Ovarian ultrasound and endoscopy for artificial insemination in dogs

Alain Fontbonne (The National Veterinary School of Alfort, France)
Catharina Linde Forsberg (Swedish University of Agricultural Sciences,Sweden)


15:30   ESDAR Board meeting (conference venue)


20:30   Welcome reception

Rectorate building, University of Cordoba, Avda. Medina-Azahara 5, 14005 - Cordoba


Friday, 28th September 2018

ESDAR Conference Sessions


08:45-09:10    Opening session. Auditorium

President of ESDAR, Rector of University of Cordoba, Chair of the local organizing committee


09:10-10:30    Plenary session I. Auditorium


09:10-09:50    Keynote lecture 1: Assisted-Reproductive Techniques in mares

Katrin Hinrichs (Texas A&M University, USA)


09:50-10:30    Keynote lecture 2: Embryo gene expression

Christine Wrenzycki (Justus Liebig University Giessen, Germany)


10:30-11:00    Coffee break


11:00-12:00    Poster session I (Posters with odd numbers)


12:00-13:15    Oral communications I: Young scientist competition
(5min presentation + 1min discussion)


Transcryptomic analysis reveals that genes related to oxidative phosphorylation are downregulated in embryos obtained with frozen thawed sperm

(J.M. Ortiz Rodriguez et al.-384, Spain)


Effects of testicular hemodynamic and echogenicity changes on ram fertilizing ability

(A.Ntemka et al.-507, Greece)


Vitrification in straws conserves motility features better than spheres in donkey

(M. Diaz-Jimenez et al.-519, Spain)


Investigating the effect of IGF-1, FSH and LH on gene expression in bovine granulosa cells

(M.J. Wieteska et al.-733, Ireland)


Gene expression and immunolocalization of β-ngf low-affinity receptor (p75) in rabbit male reproductive tract

(A.Sanchez-Rodriguez et al.-633, Spain)


Ejaculation effect on blood testosterone and prostatic PW Doppler ultrasound in dogs

(S. Alonge et al.-812, Italy)


Comparison of different sucrose-based extenders for stallion sperm vitrification

(C. Consuegra et al.-562, Spain)


Elastase inhibition affects collagen transcription and prostaglandin secretion in mare endometrium during the estrous cycle

(A. Amaral et al.-710, Portugal)


The antioxidant activity of exosomes derived from endometrial human mesenchymal stem cells improves IVF outcome in an aged murine model

(F. Marinaro et al.-406, Spain)


13:15-14:45    Lunch break

Meeting of the ESDAR Country Representatives


14:45-16:00    Oral communications II
(4 parallel sessions, 12min presentation + 3min discussion)


Session 1: Spermatology


Enrichment of a nanomicelle extender with reduced glutathione for bull semen cryopreservation

(T. Nadri et al.-726, Iran)


Age effect on semen quality, metabolomics and fertility in young Norwegian Red bulls

(B. Narud et al.-731, Norway)


Effect of protein during calcium ionophore A23187 treatment on the acrosome status of stallion sperm

(I. Ortiz et al.-749, USA)


Effect of nanowater on characteristics of deep frozen boar semen

(A. Rząsa et al.-410, Poland)


Characterization of calcium-gated channels in boar spermatozoa

(H. D. Bui et al.-459, Deutschland)



Session 2: Embryology and Obstetrics


Effect of glucose concentration during in vitro culture of equine embryo produced by ICSI

(M.J. Sánchez-Calabuig et al.-793, Spain)


Transport-related stress response in newborn foals and their dams

(C. Nagel et al.-762, Austria)


Reproductive characterization of a gray squirrel (Sciurus carolinensis) alien population: uterine scars and genital tract morphological seasonal changes

(M. Maranesi et al.-411, Italy)


Reproductive performance, endocrine and metabolic profiles in cows calving twins or singletons

(A. Waldmann et al.-417, Estonia)


Incidence of uterine torsions and the relation to blood calcium levels in Dutch dairy cows

(F. H. Jonker et al.-485, Netherlands)


Session 3: Oocyte


Lactate – a novel factor influencing the gene expression pattern in bovine granulosa cells

(A. Baufeldet al.-455, Deutschland)


Post-mortem collection, in vitro maturation and detailed assessment of caracal oocytes – a case report

(S. Prochowska et al.-521, Poland)


Omentin, new adipokine expressed in porcine ovarian follicles and regulated by progesterone hormone.

(A. Rak et al.-477, Poland)


Expression level of genes encoding antioxidant enzymes in cat (Felis domesticus) oocytes and cumulus cells during in vitro maturation (IVM)

(A. Niwinska et al.-801, Poland)


Advanced age reduces the total number of oocytes per OPU session but does not influence the blastocyst rate and embryo viability after ICSI in the mare

(J. Cuervo-Arango et al.-565, Netherlands)



Session 4: Endocrinology


Vaspin (VASP) expression is regulated by several factors in porcine ovarian follicles in vitro study

(P. Kurowska et al.-508, Poland)


Identification of microRNAs (miRNAs) predicted to regulate prostacyclin synthase (PTGIS) expression in the porcine luteal tissue

(M. Szymanska et al.-517, Poland)


The relationship between concentrations of serum IGF-I and uterine disease as well as response to synchronisation in dairy cows

(M. Beltman et al.-534, Ireland)


Expression and cellular localization of P300, CREB, SRC-1 coactivators and NCOR-2 corepressor of progesterone receptor and acetyltransferase and deacetylase activity in the reproductive system of cows

(R. Rekawiecki et al.-655, Poland)


Does early postpartum (pp) disease affect the pattern of resumption of ovulatory cycles in Holstein dairy cows?

(N.S. Ibrahim et al.-815, Scotland)


16:00-16:30    Coffee break


16:30-18:00    Parallel workshops 1-5


WS1: Embryo technologies: how do they affect embryo quality?


How vitrification affects porcine embryo quality: a key to improve vitrification outcomes – Cristina Cuello (University of Murcia, Spain)


Preimplantation embryo development in cattle: mimicking physiological conditions in vitro -Dimitrios Rizos (Institute for Agricultural and Food Research and Technology (INIA), Spain)


Optimization of protocol for medium-term storage of porcine embryos - Maria A. Gil (University of Murcia, Spain)



WS2: Artificial Insemination with frozen semen in the bitch: how to make it successful


Ovulation timing and oocyte survival: impact on canine AI with frozen semen? - Alain Fontbonne (The National Veterinary School of Alfort, France)


In situ artificial insemination in large felids: practical approach, recent advances and difficulties - Alain Fontbonne (The National Veterinary School of Alfort, France)


AI with frozen semen in the bitch - how to make it successful - Catharina Linde-Forgsberg (Swedish University of Agricultural Sciences, Sweden)


Survival and fertilizing ability of frozen-thawed canine semen after insemination - Ann Van Soom (Ghent University, Belgium)



WS3: Developmental programming and the use of epigenetics in farm animal production


Season of birth affects performance in cattle - Mieke Van Eetvelde (University of Ghent, Belgium)


Prenatal programming of commercially important traits in sheep and pigs - Cheryl Ashworth (University of Edinburgh, Scotland)


WS4: Assisted-reproductive technologies for the conservation of wildlife species


Ultra-rapid cryopreservation of sperm from wild species: an alternative for the cryopreservation in the field - J.Santiago-Moreno (Institute for Agriculture Food Research and Technology, Spain)


Studies in pampas deer (Ozotoceros bezoarticus) reproductive biology: a strategy as a base for conservation - Rodolfo Ungerfeld (Universidad de la República, Uruguay)


Critical endangered rhino species - a new rescue approach - Thomas Hildebrandt (Leibniz Institute for Zoo & Wildlife Research, Germany)


WS5: Models to predict fertility: flow cytometry analysis


Models to predict semen fertility : how can flow cytometry help us? – - Isabelle Barrier (The French Horse and Riding Institute, France)


Assess semen quality and monitor field performance: how to identify sub-fertility - Dr Marleen Broekhuijse (Utrecht University, The Netherlands)



20:30              Gala dinner



Saturday, 29th September 2018


09:00-11:30    Plenary session 2. Auditorium


09:00-09:40    Keynote lecture 3: Embryonic maternal interaction in cattle and its relation with infertility

Patrick Lonergan (University College of Dublin, Ireland)


09:40-10:20    Keynote lecture 4: Recent advances in physiology of pig reproduction

Bas Kemp (Wageningen University, The Netherlands)


10:20-10:50    Coffee break


10:50-11:30    Keynote lecture 5: Sperm cytometry analysis

Fernando Peña (University of Extremadura, Spain)


11:30-15:45    Oral communications III

(4 parallel sessions, 12min presentation + 3min discussion)


Session 5: Embryology and Gynaecology


On-farm ovum pick-up in pregnant Holstein cows and in vitro production of embryos in Estonia

(E. Mark et al.-586, Estonia)


IGF- binding protein-2 concentration is higher in cows suffering from embryonic/early fetal mortality

(L. Jessen et al.-554, Deutschland)


How ram epididymal and electroejaculated sperm differ in their behavior during capacitation: impact on cleavage and blastocyst development

(O. Garcia Alvarez et al.-664, Spain)


Generation of TPC2 knock out pig embryos by CRISPR-Cas technology

(J. Gadea et al.-725, Spain)


Comparison of conventional sediment smears and cytospins in low volume uterine lavage – a study in 16 mares (R. Marcos et al.-512, Portugal)


Session 6: Spermatology


Effect of ultra-low concentrations of benzimidazole derivative on equine sperm resistance to freezing

(E. Nikitkina et al.-432, Russia)


Cryopreservation of rhinoceros sperm                                                                                                                                    

(R. Hermes et al.-442, Ireland)


Isolation and characterization of cysteine-rich venom protein-like isoform X2 from turkey (Meleagris galopavo) seminal plasma

(M. Słowińska et al.-610, Poland)


Improvement of canine spermatozoa vitrification

(J. Cerdeira et al.-705, Spain)


Effect of different seminal plasma fractions on viability of jack spermatozoas and on its interaction with polymorphonuclear cells

(M. Papas et al.-660, Spain)


Session 7: Gynaecology


Bovine Viral Diarrhoea Virus Infection Interrupts the Regulatory Pathways for Uterine Interferon Stimulated Gene Expression in Cows

(Z. Cheng et al.-452, United Kingdom)


Fibrosis in donkey endometrium: how can we interpret it?

(J. Aguiar Silva et al.-707, Portugal)



DNA methylation in equine endometrium with different degrees of fibrosis

(J. Alpoim-Moreira et al.-741, Portugal)


Assessment of automatic quantitative analysis of slides to fast endometritis evaluation in mares

(L. Zdrojkowski et al.-767, Poland)


Non-esterified fatty acids (NEFAs) increase the production of IL-6 and IL-8 in bovine endometrial epithelial cells (bEECs) in culture

(W. Chankeaw et al.-739, Poland)



Session 8: Endocrinology


Immune checkpoint control of corpus luteum: expression profile of PD-1, PD-L1, and CTLA4 mRNAs in the ovine corpus luteum during early pregnancy and prostaglandin F2α-induced luteolysis

(M. Hitit et al.-722, Turkey)


Search for a pleiotropic effect of the FecLL prolific mutation in Lacaune meat sheep

(L. Chantepie et al.-650, France)


Maternal and fetal hair cortisol concentrations in beef cattle at delivery

(T. Peric et al.-654, Slovenia)


The effect of GnRH on the endometrial expression of oestrogen receptors in dairy cows

(M. Trela et al.-783, Poland)


NGF system is differentially expressed in the ovary, oviduct and uterus of rabbit does although independent of serum hormonal levels

(R.M. Garcia Garcia et al.-504, Spain)


12:45-13:15    ESDAR annual business meeting


13:15-14:45    Lunch break


14:45-15:45    Poster session II (posters with even numbers)


15:45-16:15    Coffee break


16.15-17:45    Parallel workshops 6-10

WS6: Novel fertility traits and genomic markers in dairy cows


Novel fertility traits and genomic markers in dairy cows - Mark Crowe (University College of Dublin, Ireland)


Metabolic clustering of dairy cows at early and peak lactation - Jenne de Koster (Ghent University, Belgium)


Genomic markers derived from novel milk mid-infrared spectra based fertility traits - Nicolas Gengler (University of Liege, Belgium)



WS7: Assisted-reproductive techniques in cats applied to wild felids conservation


Assisted Reproductive Techniques (ART) in rescue programs of endangered wild felids-possibilities, obstacles and challenges – Wojciech Nizanski (Wroclaw University of Environmental and Life Sciences, Poland)


Fertility preservation in felids - Gaia Luvoni (Università degli Studi di Milano, Italy)


WS8: Sperm selection


Sperm selection for assisted reproduction – Jane Morrell (Swedish University of Agricultural Sciences, Sweden)


Use of Single Layer Centrifugation to select sperm in dromedary camel semen - Clara Malo (Camel Reproduction Center (RDC), United Arab Emirates)


WS9: The use of Anti-Müllerian hormone in fertility management


Anti-Müllerian hormone as a predictor of fertility in cattle - Francesca Mossa (University of Sassari, Italy)


Dysregulation of the anti-Müllerian hormone and its specific receptor by LH and steroids may contribute to their overexpression in granulosa cells from women with polycystic ovary syndrome - Nathalie Di Clemente (Paris Diderot University, France)


Relationship between circulating AMH and puberty, fertility and prolificacy of small ruminants - Stephane Fabre (Université de Toulouse, INRA, ENVT, France)


WS10: Endometritis/Endometrosis in the mare: applications of stem cells


Evidence-based treatment of persistent post-breeding endometritis in mares - Terttu Katila(University of Helsinki, Finland)


Can cell-free products secreted by stem cell promote endometrial regeneration? - Anna Consiglio-Lange (Università degli Studi di Milano, Italy)


17:45              Closure of ESDAR


21.00              Equestrian show




Important deadlines:

Abstract submission: opens 15th January – closes 1st March

Early bird registration: 1st July